Development And Validation Of UV–Spectrophotometric Methods For Estimation Of Ceftriaxone In Bulk And Tablet Dosage Form

Ceftriaxone is a third-generation cephalosporin antibiotic. Like other third-generation cephalosporins, it has broad spectrum activity against Gram negative and Gram positive bacteria Various methods for analysis of the same are available but are time consuming and expensive. Here we have developed two new, precise and simple UV spectrophotometric methods for estimation of ceftriaxone from bulk and tablet formulation in phosphate buffer 7.4. The drug obeyed the Beer’s law with correlation coefficient 0.996 and 0.998 respectively for Method I and Method II. It showed absorption maxima at 340 nm and 360 nm respectively for method I and Method II; in phosphate buffer 7.4. The linearity was observed between 5 –40 μg/ml. The results of analysis were validated by recovery studies, accuracy, precision, LOD, LOQ and ruggedness. The method was found to be simple, accurate, precise, economical and robust.